OPLAH CLIA Kit for Bovine(Bos taurus) | EIAab.comOPLAH CLIA Kit for Bovine(Bos taurus) | EIAab.com

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OPLAH (Gene name),

5-oxoprolinase (Protein name ), OPLA_BOVIN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

OPLAH;

Protein name:

5-oxoprolinase;

Alternative:

Pyroglutamase;5-oxo-L-prolinase(5-OPase);

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

Homodimer.

Function:

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

1288

Sequence:

50:

MGGPEGRFHF | AIDRGGTFTD | VFAQCPGGHV | RVLKLLSEDP | ANYVDAPTEG |

100:

IRRILEQEGG | VLLPRDRPLD | TSRIASIRMG | TTVATNALLE | QQGERVALLV |

150:

TRGFRDLLHV | CTQARAXLFD | LAVPMPETLY | EEVLEVDERV | VLYRGXPGAG |

200:

TPVKGCTGDL | LEVQQPVDLG | GLRWKLEGLL | SRGIRSLAVV | LMHSYTWAQH |

250:

EQQVGALARE | LGFTHVSLSS | EAMPMVRIVP | RGHTACADAY | LTPTIQRYVQ |

300:

GFRRGFQGQL | KDVQVLFMRS | DGGLAPMDSF | SGSRAVLSGP | AGGVVGYSAT |

350:

TYRVEGGQPV | IGFDMGGTST | DVSRYAGEFE | HVFEASTAGV | TLQAPQLDIN |

400:

TVAAGGGSRL | FFRSGLFVVG | PESAGAHPGP | ACYRKGGPVT | VTDANLVLGR |

450:

LLPASFPCIF | GPGEDQPLSP | EASRKALEAV | ATEVNSFLTN | GPCPASPLSL |

500:

EEVAMGFVRV | ANEAMCRPIR | ALTQARGHDP | SAHVLACFGG | AGGQHACAIA |

550:

RALGMDTVHI | HRHSGLLSAL | GLALADVVHE | AQEPCSLPYA | PETFAQLDQR |

600:

LGRLEEQCVE | ALRAQGFPRS | QISTESFLHL | RYQGTDCALM | VSAHQHPASA |

650:

RSPRAGDFGA | AFVERYMREF | GFIIPERPVV | VDDVRVRGTG | SSSLRLEDVP |

700:

KAHSGPPRVD | KMTQCYFEGG | YQETPVYLLG | ELGCGHKLQG | PCLIIDSNST |

750:

ILVEPGCQAE | VTETGDIRIS | VGAETASVVG | TQLDPIHLTI | FSHRFMSIAE |

800:

QMGRILQRTA | ISTNIKERLD | FSCALFGPDG | GLVSNVPHIP | VHLGAMQETV |

850:

QFQIQQLGAD | LHPGDVLLSN | HPSAGGSHLP | DLTVITPVFW | PGQTRPVFYV |

900:

ASRGHHADIG | GITPGSMPPH | STSLQQEGAV | FLSFKLVHGG | VFQEEAVTEA |

950:

LRAPGKIPGC | SGTRNLHDNL | SDLRAQVAAN | QKGIQLVGEL | IGQYGLDVVQ |

1000:

AYMGHIQANA | ELAVRDMLRA | FGTARQARGL | PLEVSAEDHM | DDGSPIRLRV |

1050:

QINMSQGSAV | FDFSGSGPEV | FGNLNAPRAI | TLSALIYCLR | CLVGRDIPLN |

1100:

QGCLAPVRVV | IPKGSILDPS | PDAAVVGGNV | LTSQRVVDVI | LGAFGACAAS |

1150:

QGCMNNVTLG | NAHMGYYETV | AGGAGAGPGW | HGRSGVHSHM | TNTRITDPEI |

1200:

LESRYPVILR | RFELRLGSGG | RGRFRGGDGI | IRELLFREEA | LLSVLTERRA |

1250:

FQPYGLMGGE | PGARGLNLLI | RKDGRTVNLG | GKTSVPVYPG | DVFCLHTPGG |

1288:

GGYGDPGDPA | PLPGSPLQPL | AFPERGSVYE | YRRAQEAV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Pfam:

STRING:

9913.ENSBTAP00000022981

KEGG:

UniGene:

Uniprot:

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ELISA Kit for Bovine 5-oxoprolinase

Cat.:

E6081m

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Packing:

96T

ELISA Kit for Bovine 5-oxoprolinase

Cat.:

E6081r

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Packing:

96T

ELISA Kit for Bovine 5-oxoprolinase

Cat.:

E6081h

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ELISA Kit for Bovine 5-oxoprolinase

Cat.:

E6081b

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96T

CLIA Kit for Bovine 5-oxoprolinase

Cat.:

U6081m

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96T

CLIA Kit for Bovine 5-oxoprolinase

Cat.:

U6081r

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96T

CLIA Kit for Bovine 5-oxoprolinase

Cat.:

U6081h

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96T

CLIA Kit for Bovine 5-oxoprolinase

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U6081b

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96T

Polyclonal Antibody for Bovine 5-oxoprolinase

Cat.:

P6081Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Bovine 5-oxoprolinase

Polyclonal Antibody for Bovine 5-oxoprolinase

Polyclonal Antibody for Bovine 5-oxoprolinase

Cat.:

P6081Rb-m

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Monoclonal Antibody for Bovine 5-oxoprolinase

Monoclonal Antibody for Bovine 5-oxoprolinase

Monoclonal Antibody for Bovine 5-oxoprolinase

Monoclonal Antibody for Bovine 5-oxoprolinase

Protein for Bovine 5-oxoprolinase

Protein for Bovine 5-oxoprolinase

Protein for Bovine 5-oxoprolinase

Protein for Bovine 5-oxoprolinase

R&D Technical Data

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

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Recovery

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Recovery was determined by spiking various levels of OPLA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	94	91-100
Plasma	96	93-103

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPLA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-100%	88-101%	91-97%	92-98%
EDTA plasma(n=5)	89-105%	90-102%	88-103%	90-104%
heparin plasma(n=5)	93-101%	94-99%	92-100%	91-101%

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References

1.

"Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery."
Watanabe T. , Abe K. , Ishikawa H. , Iijima Y.
Biol. Pharm. Bull.27:288-294(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 3-14;TISSUE SPECIFICITY
tissue: Kidney.

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